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    10X Genomics cell segmentation kits 10x genomics
    Cell Segmentation Kits 10x Genomics, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+segmentation+kits+10x+genomics/10x+cell+onboard+segmentation+xenium/pm40112802-236-228-231
    Average 86 stars, based on 1 article reviews
    cell segmentation kits 10x genomics - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Recombinant:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Electron Microscopy:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Imaging:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    TUNEL Assay:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    RNAscope:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Multiplex Assay:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Fluorescence:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Gene Expression:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    Isolation:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    In Situ:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    RNA Sequencing:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article

    FACS:

    Article Title: Growth of the maternal intestine during reproduction.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER D-(+)-Glucose Sigma-Aldrich Cat#G8270 D-( )-Fructose Sigma-Aldrich Cat#F0127 Sodium Chloride Sigma-Aldrich Cat#S7653 Sucrose Sigma-Aldrich Cat#S1888 DAPI Thermo Scientific Cat#62248 Matrigel, Growth Factor Reduced Corning Cat#356231 DMEM/F-12 with 15 mM HEPES STEM CELL Technologies Cat#36254 Penicillin and Streptomycin VWR Cat#392-0406 GlutaMAX Supplement Gibco Cat#35050061 B-27 Supplement Gibco Cat#17504001 N-2 Supplement Gibco Cat#17502048 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A7250 R-Spondin Novartis N/A Mouse Noggin Recombinant Protein Gibco Cat#250-38 Mouse EGF Recombinant Protein R&D Systems Cat#2028-EG-200 Paraformaldehyde 16% Aqueous Solution Electron Microscopy Sciences Cat#15710-S Normal Donkey Serum Sigma-Aldrich Cat#S30-M Mouse Prolactin Recombinant Protein Gibco Cat#315-16 Critical commercial assays Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 488 dye Invitrogen Cat#C10637 Click-iT Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor 647 dye Invitrogen Cat#C10640 DISCOVERY ChromoMap DAB Kit (RUO) Roche Cat#760-159 DeadEnd Colorimetric TUNEL System Kit Promega Cat#G7130 RNAscope LS Multiplex Fluorescent Assay Bio-Techne Cat#322800 RNeasy Plus Micro Kit Qiagen Cat#74034 RNeasy Plus Mini Kit Qiagen Cat#74134 RNeasy Mini Kit Qiagen Cat#74104 SuperScript VILO Master Mix Invitrogen Cat#11766050 TaqMan Gene Expression Master Mix Applied Biosystems Cat#4369510 T7 mMessage mMachine Kit Invitrogen Cat#AM1344 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs Cat#E7760 NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490 Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics Cat#1000121 Xenium In Situ Gene Expression with Cell Segmentation Kits 10x Genomics Cat#1000460; Cat#1000487; Cat#1000661 Deposited data Small intestine bulk RNA sequencing This paper GEO: GSE247929, GSE247926 FACS-sorted intestinal epithelium bulk RNA sequencing, lactating This paper GEO: GSE247929, GSE247923 FACS-sorted intestinal epithelium bulk RNA sequencing, pregnancy This paper GEO: GSE247929, GSE247925 FACS-sorted intestinal epithelium singlecell RNA sequencing This paper GEO: GSE247929, GSE247927 Gut rolls Xenium in situ spatial imaging analysis This paper GEO: GSE285027 (Continued on next page) ll OPEN ACCESS Cell 188, 1–19.e1–e10, May 15, 2025 e2 Please cite this article in press as: Ameku et al., Growth of the maternal intestine during reproduction, Cell (2025), https://doi.org/10.1016/ j.cell.2025.02.015 Article



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